Nutrient Agar

Nutrient agar is a basic medium and commonly used for cultivation, enrichment, enumeration, prolonged maintenance and storage of wide variety of microbes. The nutrient agar is widely used as non-selective microbial medium for routine microbiology analysis. Nutrient agar is prominently used for cultivation of the non-fastidious organisms. Nutrient agar is ideal microbial media for the teaching, demonstration and routine analysis. Nutrient Agar is a simple medium composed of the peptone, meat extract (equivalent to beef extract), yeast extract which provide ample amount of the carbon and nitrogen essential and non essential amino acids, along with the vitamins and trace minerals. Sodium chloride maintains the osmotic equilibrium of the medium. Agar is solidifying agent. Use: Recommended for the refinement of non-fastidious microorganisms from clinical and non-clinical samples. It is generally used for examination of water and dairy products. Contents* Ingredients Peptone Meat extract # Yeast extract Sodium chloride Agar pH at 25˚C Gram/Liter 5.0 1.5 1.5 5.0 15.0 7.4±0.2 * Formula adjusted for optimum performance and parameters # Equivalent to Beef Extract Directions: Dissolve 28.00 grams in 1000 ml distilled water. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C and distribute aseptically in desired. Ensure complete solidification and inoculate test sample aseptically.