
Dog Relaxin Pregnancy Test
In Vitro Diagnostic Tests
Information
Canine Relaxin Pregnancy Rapid Test Cassette (Whole Blood/Serum/Plasma)
Intended Use:
The Canine Relaxin Pregnancy Test is a qualitative immunochromatographic assay designed to detect relaxin, a hormone produced by the placenta, as an early indicator of pregnancy in dogs. For veterinary diagnostic use by professionals.
Principle:
Relaxin is a hormone secreted by the developing placenta of pregnant dogs. It becomes detectable in blood from approximately 15 days post-ovulation or 15–18 days post-breeding. This rapid test uses high-affinity monoclonal antibodies specific to canine relaxin, allowing for quick and reliable visual results.
Kit Contents:
• 1x Test cassette (foil pouch with desiccant)
• 1x Dropper or capillary sampler
• 1x Dilution buffer
• 1x Alcohol pad (optional)
• 1x Instructions for use
Storage: Store at 2–30°C. Do not freeze.
Shelf Life: 18–24 months from the manufacturing date.
Sample Requirements:
• Acceptable samples: Whole blood (fresh or anticoagulated), serum, or plasma
• Recommended volume: 3–5 µL
• Use immediately or refrigerate at 2–8°C for up to 24 hours
Test Procedure:
1. Sample Collection
• Collect blood from a peripheral vein (e.g., cephalic or saphenous)
• Allow to clot for serum, or centrifuge for plasma if needed
2. Test Execution
• Open foil pouch and place the cassette on a flat surface
• Add 3–5 µL of sample into the sample well (S)
• Immediately add 2–3 drops (approx. 100 µL) of the buffer solution
3. Result Reading
• Wait 10–15 minutes
• Do not read results after 20 minutes
Result Interpretation:
• ✅ Positive: Two colored lines (Control “C” + Test “T”) appear
→ Pregnant (Relaxin detected)
• ❌ Negative: Only the Control “C” line appears
→ Not pregnant or test done
